Excess stain was removed by soaking the gels in metha- nol/acetic acid/water ( 30 : 10 : 60) then in methanol/acetic acid/water (5:7: 88). If protein staining ... according to the manufacturera#39;s instructions, soaked in 5% glycerol, and then dried under vaccuum at 60AdC for 2 h followed by 2 h at room temperature. Kodak XAR-5 or AGFA CURIX RP-1 film was used and exposures were carried out at - 70AdC. 2.7.
Title | : | Advances in Environmental Science and Technology |
Author | : | |
Publisher | : | - 1988 |
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